Modification of the Dispersion Curve Shape of Liver Tissue Conductivity under the Influence of Destructive Factors

Editorial Board PCSS Journal; D.M. Chervinko; B.I. Rachiy; L.S. Yablon; V.O. Kotsiubynsky; O.P. Pakhovsky; I.M. Hasiuk

doi:10.15330/pcss.26.3.541-548

Authors

D.M. Chervinko Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine
B.I. Rachiy Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine
L.S. Yablon Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine
V.O. Kotsiubynsky Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine
O.P. Pakhovsky Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine
I.M. Hasiuk Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine

DOI:

https://doi.org/10.15330/pcss.26.3.541-548

Keywords:

impedance spectroscopy, electrical conductivity dispersion, liver tissues, electrical equivalent circuit, destruction, alpha-dispersion, numerical differentiation, biological tissues, cell membranes

Abstract

This work investigates the frequency dispersion of electrical properties of biological tissues, particularly pig liver, using spectral analysis of conductivity. Numerical differentiation of impedance characteristics was applied over a wide frequency range (0.01 Hz – 100 kHz) to identify local transitions between tissue electrophysiological regimes. It is shown that the position of the local minimum of the first derivative of the imaginary part of specific conductivity near 1 Hz, corresponding to alpha-dispersion and interfacial polarization on cell membranes, remains stable regardless of sample size but shifts sensitively under the influence of destructive factors (e.g., temperature, radiation). These results confirm the feasibility of using conductivity derivatives as an informative marker of structural changes in tissues, complementing classic impedance approaches for diagnosing and monitoring pathological processes.

References

S. Emran, R. Lappalainen, A.M. Kullaa, & S. Myllymaa, Concentric Ring Probe for Bioimpedance Spectroscopic Measurements: Design and Ex Vivo Feasibility Testing on Pork Oral Tissues. Sensors, 18(10), 3378 (2018); https://doi.org/10.3390/s18103378.

M. Grossi, and B. Riccò, Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review, J. Sens. Sens. Syst., 6, 303 (2017); https://doi.org/10.5194/jsss-6-303-2017.

Shi, Yueying & Bai, Xiaoxiao & Yang, Jingrong & Wu, Xinyu & Wang, Lei. Optimized measurement methods and systems for the dielectric properties of active biological tissues in the 10Hz-100 MHz frequency range. Frontiers in Physiology. 16 (2025); https://doi.org/10.3389/fphys.2025.1537537.

S, El. The Use of Electrical Impedance Spectroscopy for Medical Application: A Mini Review. Physical Science & Biophysics Journal. 7, 1 (2022); https://doi.org/10.23880/psbj-16000250.

D.A. Dean, T. Ramanathan, D. Machado, & R. Sundararajan, Electrical Impedance Spectroscopy Study of Biological Tissues. Journal of electrostatics, 66(3-4), 165 (2008); https://doi.org/10.1016/j.elstat.2007.11.005.

Pinto, Sandra & Pinzón, Edgar & Melendez, Angel & Mendez, Stelia & Miranda, David. Limpieza de electrodos y reproducibilidad de medidas de impedancia eléctrica en células HeLa en solución acuosa. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales. 44, 257 (2020); https://doi.org/10.18257/raccefyn.919.

Dean, David & Thillaiyan, Ramanathan & Machado, Diego & Sundararajan, Raji. Electrical Impedance Spectroscopy Study of Biological Tissues. Journal of electrostatics. 66, 165 (2008); https://doi.org/10.1016/j.elstat.2007.11.005.

T. Gerasimenko, S. Nikulin, G. Zakharova, A. Poloznikov, V. Petrov, A. Baranova, & A. Tonevitsky, Impedance Spectroscopy as a Tool for Monitoring Performance in 3D Models of Epithelial Tissues. Frontiers in bioengineering and biotechnology, 7, 474 (2020); https://doi.org/10.3389/fbioe.2019.00474.

Van Haeverbeke, Maxime & De Baets, Bernard & Stock, Michiel. Plant impedance spectroscopy: a review of modeling approaches and applications. Frontiers in Plant Science. 14 (2023); https://doi.org/10.3389/fpls.2023.1187573.

Bertemes Filho, Pedro & Nogueira Cavalieri, Ricardo. Plant tissue differentiation using electrical impedance spectroscopy with deep neural networks, International Journal of Biosensors & Bioelectronics. 6, (2020); https://doi.org/10.15406/ijbsbe.2020.06.00182.

Yao, Jiafeng & Wang, Li & Liu, Kai & Wu, Hongtao & Jingshi, Huang & Li, Jianping. Evaluation of Electrical Characteristics of Biological tissue with Electrical Impedance Spectroscopy. Electrophoresis., 41(16-17), 1425 (2020); https://doi.org/10.1002/elps.201900420.

G.M.G. da Silva, P.M. Faia, S.R. Mendes, & E.S. Araújo, A Review of Impedance Spectroscopy Technique: Applications, Modelling, and Case Study of Relative Humidity Sensors Development. Applied Sciences, 14(13), 5754 (2024); https://doi.org/10.3390/app14135754.

H.S. Magar, R.Y.A. Hassan, & A. Mulchandani, Electrochemical Impedance Spectroscopy (EIS): Principles, Construction, and Biosensing Applications. Sensors, 21(19), 6578 (2021); https://doi.org/10.3390/s21196578.

Тaras Pryimak, Oksana Popadynets, Ivan Gasiuk, Taras Kotyk. Electrical impedance spectrum transformation of liver tissues under the influence of temperature. International Journal of Engineering Research and Applications, 11(12), 1 (2021); https://doi.org/10.9790/9622-1112010111.

J. Hou, R. Strand-Amundsen, S. Hødnebø, T.I. Tønnessen, & J.O. Høgetveit, Assessing Ischemic Injury in Human Intestine Ex Vivo with Electrical Impedance Spectroscopy. Journal of electrical bioimpedance, 12(1), 82 (2021); https://doi.org/10.2478/joeb-2021-0011.

S.M. Abie, Ø.G. Martinsen, B. Egelandsdal, J. Hou, F. Bjerke, A. Mason, & D. Münch, Feasibility of Using Electrical Impedance Spectroscopy for Assessing Biological Cell Damage during Freezing and Thawing. Sensors (Basel, Switzerland), 21(12), 4129 (2021); https://doi.org/10.3390/s21124129.

S. Abasi, J.R. Aggas, G.G. Garayar-Leyva, B.K. Walther, & A. Guiseppi-Elie, Bioelectrical Impedance Spectroscopy for Monitoring Mammalian Cells and Tissues under Different Frequency Domains: A Review. ACS measurement science au, 2(6), 495 (2022); https://doi.org/10.1021/acsmeasuresciau.2c00033.

Y. Guo, W. Wang, W. Li, J. Li, M. Zhu, R.nSong, W. Zhu, L. Wang, Z. Ji, & X. Shi, In vivo electrical properties of the healthy liver and the hepatic tumor in a mouse model between 1 Hz and 1 MHz during a thermal treatment. International journal of hyperthermia: the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, 41(1), 2396122 (2024); https://doi.org/10.1080/02656736.2024.2396122.

S. Prakash, M.P. Karnes, E.K. Sequin, J.D. West, C.L. Hitchcock, S.D. Nichols, M. Bloomston, S.R. Abdel-Misih, C.R. Schmidt, E.W. Martin, S.P. Jr, Povoski, & V.V. Subramaniam, Ex vivo electrical impedance measurements on excised hepatic tissue from human patients with metastatic colorectal cancer. Physiological measurement, 36(2), 315 (2015); https://doi.org/10.1088/0967-3334/36/2/315/.

S. Fuentes-Vélez, S. Fagoonee, A. Sanginario, M. Pizzi, F.nAltruda, & D. Demarchi, Electrical Impedance-Based Characterization of Hepatic Tissue with Early-Stage Fibrosis. Biosensors, 12(2), 116 (2022); https://doi.org/10.3390/bios12020116.

E. Priidel, P. Annus, A. Krivošei, M. Rist, R. Land, M. Min, & O. Märtens, Methods for Detection of Bioimpedance Variations in Resource Constrained Environments. Sensors (Basel, Switzerland), 20(5), 1363 (2020); https://doi.org/10.3390/s20051363.

R.P. Jokhi, V.V. Ghule, B.H. Brown, & D.O. Anumba, Reproducibility and repeatability of measuring the electrical impedance of the pregnant human cervix-the effect of probe size and applied pressure. Biomedical engineering online, 8, 10(2009); https://doi.org/10.1186/1475-925X-8-10.

V. Garr Barry, J.L. Chiang, K.G. Bowman, K.D. Johnson, & B.A. Gower, Bioimpedance-Derived Membrane Capacitance: Clinically Relevant Sources of Variability, Precision, and Reliability. International journal of environmental research and public health, 20(1), 686 (2022); https://doi.org/10.3390/ijerph20010686.

S. Kophamel, L.C. Ward, E. Ariel, D. Mendez, L.M. O'Brien, L. Burchell, & S.L. Munns, A Standardized Protocol for Measuring Bioelectrical Impedance in Green Turtles (Chelonia mydas). Physiological and biochemical zoology: PBZ, 96(2), 87 (2023); https://doi.org/10.1086/722451.

K. Kulkeaw, & W. Pengsart, Progress and Challenges in the Use of a Liver-on-a-Chip for Hepatotropic Infectious Diseases. Micromachines, 12(7), 842 (2021); https://doi.org/10.3390/mi12070842.

Grimnes, Sverre & Martinsen, Ørjan. Alpha-dispersion in human tissue. Journal of Physics: Conference Series. 224, 012073 (2010); https://doi.org/10.1088/1742-6596/224/1/012073.