Effects of Sodium Nitroprusside on Salt Stress Tolerance of Tocopherol-Deficient Arabidopsis Thaliana Plants

Abstract

In the present study, effects of exogenous sodium nitroprusside (SNP), a nitric oxide
(•NO) donor, on lipid peroxidation and antioxidant enzyme activities in wild type and tocopheroldeficient lines vte1 and vte4 of Arabidopsis thaliana subjected to 200 mM NaCl were studied. In wild
type plants, pretreatment with SNP did not change level of thiobarbituric acid reactive substances
(TBARS), but decreased the activities of dehydroascorbate reductase and guaiacol peroxidase
under salt stress. In mutant line vte1, which lacks all forms of tocopherols, pretreatment with SNP
reduced TBARS level and increases the activities of glutathione reductase and guaiacol peroxidase
under salt stress. Ascorbate peroxidase activity decreased under salt stress conditions in both
mutant lines, pretreated with SNP. It can be concluded, that pretreatment with SNP could
attenuate salt-induced injuries in A. thaliana plants via up-regulation of activity of antioxidant
enzymes and attenuate lipid peroxidation