Abnormalities of Centromeres and Carcinogenesis of Non-Hodgkin's Lymphoma in Children
DOI:
https://doi.org/10.15330/jpnubio.10.96-106Keywords:
non-Hodgkin's lymphoma, C-anaphase, premature centromere division, metaphase chromosomes, treatment strategies, pediatric casesAbstract
This article presents the results of long-term studies (1991–2023) of the relationship between abnormal behavior of the centromere, in particular, the phenomena of premature centromere division of metaphase chromosomes (PCD) and premature anaphase of metaphase chromosomes (C-anaphase), and the pathogenesis of non-Hodgkin lymphomas (NHL) in children. 17 children with B-form non-Hodgkin's lymphoma were studied before the start of treatment in the first acute period. The studies were aimed at the manifestation and levels of these phenomena during the first acute period of B-form non-Hodgkin's lymphoma in peripheral blood and bone marrow cell culture in children. The study found compelling evidence, supported by statistical analysis, indicating that C-anaphase occurs with an extremely high frequency in children diagnosed with non-Hodgkin's lymphoma, both in the bone marrow and in the peripheral blood. C-anaphase levels in children with non-Hodgkin's lymphoma were 51.27 ± 1.99% in peripheral blood and 67.47 ± 2.19% in red bone marrow. At the same time, this phenomenon did not occur at all in the control group - normal healthy donors and healthy children neither in the peripheral blood nor in the red bone marrow (0%). These data allow us to propose the use of C-anaphase as an additional diagnostic criterion for the detection of non-Hodgkin's lymphoma, especially in the early stages of the disease. Further study of the mechanisms underlying centromere abnormalities may reveal new mechanisms of the appearance, development and pathogenesis of non-Hodgkin's lymphoma, paving the way for targeted therapeutic interventions and personalized treatment strategies, and elucidate the mechanisms of new forms of programmed cell death in various pathologies. Our studies allow us to draw a conclusion about the relationship between centromere abnormalities and the pathogenesis of non-Hodgkin's lymphoma, about the importance of determining the level of C-anaphase as a diagnostic indicator, and its potential role in the study of both pathogenesis and clinical practice related to this hematological malignancy.
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